Glucose 6-Phosphate Dehydrogenase Enzyme Activity, Blood
Use
The test is useful for evaluating individuals with episodic or chronic Coombs-negative non-spherocytic hemolytic anemia, assessing glucose 6-phosphate dehydrogenase (G6PD) enzyme capacity prior to therapies like rasburicase that may cause hemolysis or methemoglobinemia in G6PD deficient patients, and contributing to the creation of a comprehensive patient profile to ensure appropriate monitoring for developing anemia.
Special Instructions
Ensure to send the whole blood specimen in the original tube and avoid aliquoting. If not ordering electronically, complete and include the Benign Hematology Test Request (T755) with the specimen.
Limitations
Normal glucose 6-phosphate dehydrogenase (G6PD) activity values may be recorded during hemolytic events for several weeks following hemolysis. Reticulocytosis from any cause can increase the activity level, potentially masking some G6PD deficiency cases. Recent blood transfusions may obscure intrinsic enzyme activity and skew results. High white blood cell counts may interfere with the test and falsely elevate G6PD activity, although this varies with the type of white blood cell.
New York Approved
Methodology
Automated Analyzer (Clinical Chemistry)
Biomarkers
LOINC Codes
- 32546-4
- 32546-4
Result Turnaround Time
1-4 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
6 mL
Minimum Volume
1 mL
Container
Yellow top (ACD solution B) preferred; Lavender top (EDTA) or yellow top (ACD solution A) acceptable
Collection Instructions
Send whole blood specimen in original tube. Do not aliquot.
Causes for Rejection
Gross hemolysis, fully clotted
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 20 days |