Use

This test is useful for evaluating individuals with Coombs-negative chronic hemolysis as part of a profile. It measures the activity of the glucose phosphate isomerase (GPI) enzyme, which is crucial in the glycolysis pathway, converting glucose 6-phosphate to fructose 6-phosphate. GPI deficiency is linked to nonspherocytic hemolytic anemia, which can vary from mild to severe anemia and may include jaundice, gallstones, and splenomegaly. In some cases, it leads to neonatal death or hydrops fetalis. Rarely, it can also involve neurological defects and granulocyte dysfunction.