Glutamine Synthetase Immunostain, Technical Component Only
Use
This test is useful for the classification of hepatic adenomas and the identification of focal nodular hyperplasia. Glutamine synthetase (GS) is an enzyme that catalyzes the adenosine triphosphate-dependent condensation of glutamate with ammonia to form glutamine. It is expressed in hepatic adenomas with beta-catenin alterations and in zone 3 of normal liver, with a characteristic map-like pattern in focal nodular hyperplasia.
Special Instructions
This test includes only the technical performance of the stain; no pathologist interpretation is provided. If pathologist interpretation is needed, order PATHC/Pathology Consultation. Technical component only stains should not be ordered with PATHC, as they will be canceled.
Limitations
Age of a cut paraffin section can affect immunoreactivity, with stability thresholds varying widely. Environmental factors can affect the charge of glass slides, potentially altering slide staining. Unsuitable glass slides may reduce staining consistency.
FDA ApprovedNew York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- Order only;no result
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Preferred: Formalin-fixed, paraffin-embedded tissue block OR 2 unstained, positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.
Storage Instructions
Attach the appropriate labels included in the kit to the transport container.
Causes for Rejection
Wet/frozen tissue, cytology smears, nonformalin fixed tissue, nonparaffin embedded tissue, noncharged slides, ProbeOn slides, Snowcoat slides
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |
| Refrigerated | Refrigerated |