Glypican-3 Immunostain, Technical Component Only
Use
Glypican-3 (GPC3) protein is a member of the glypican family of heparin sulfate proteoglycans that are phosphatidylinositol-anchored to the cytoplasmic membrane. GPC3 acts as a coreceptor for heparin-binding growth factors, which play an important role in cell growth and differentiation. Diagnostically, GPC3 will aid in separating hepatocellular carcinomas from other malignancies and hepatic adenomas. It is expressed in 70% to 90% of hepatocellular carcinomas.
Special Instructions
Not provided.
Limitations
Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen dependent. Best practice is for paraffin sections to be cut within 6 weeks. The charge of glass slides can be affected by environmental factors and subsequently may alter slide staining. Sending unsuitable glass slides can result in inconsistent staining due to poor slide surface chemistry.
FDA ApprovedNew York Approved
Methodology
Immunoassay (IHC)
Biomarkers
Glypican-3
Protein
LOINC Codes
- Order only;no result
- Bill only; no result
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Formalin-fixed, paraffin-embedded tissue block or 2 unstained, positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.
Causes for Rejection
Wet/frozen tissue, Cytology smears, Nonformalin fixed tissue, Nonparaffin embedded tissue, Noncharged slides, ProbeOn slides, Snowcoat slides
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |