Hemoglobin (Hb) Immunostain, Technical Component Only
Use
This test is used for the identification of red blood cells and red blood cell precursors. It utilizes an immunostain with antibodies to hemoglobin that stains red blood cells and their precursors in bone marrow and in other sites in a diffuse cytoplasmic staining pattern. It may be helpful in diagnosing erythroleukemia or myelodysplastic neoplasms.
Special Instructions
This test includes only the technical performance of the stain; no pathologist interpretation is provided. Technical component only stains should not be ordered with PATHC / Pathology Consultation. If ordered with PATHC, the technical component stains will be canceled. Clients wishing to access digital images must order IHC stains electronically.
Limitations
Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen dependent. Best practice is for paraffin sections to be cut within 6 weeks. This test does not include pathologist interpretation, only technical performance of the stain, and should be interpreted in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- Order only;no result
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container. Preferred: 2 Unstained positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick. Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block.
Causes for Rejection
Wet/frozen tissue, Cytology smears, Nonformalin fixed tissue, Nonparaffin embedded tissue, Noncharged slides, ProbeOn slides
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |