Hepatitis B Virus e Antibody, Serum
Use
This test is useful for determining the presence or absence of detectable hepatitis B virus e antibody (anti-HBe) in monitoring the infection status of individuals with chronic hepatitis B. It helps in determining the infectivity of hepatitis B virus (HBV) carriers and in monitoring serologic response of chronically HBV-infected patients receiving antiviral therapy. The presence of anti-HBe usually indicates inactivity of the virus and low infectivity in HBV carriers. Positive results should be interpreted in conjunction with other clinical and laboratory findings.
Special Instructions
For optimal results, patients should not take multivitamins or dietary supplements containing biotin (vitamin B7) 24 hours prior to specimen collection. This test is part of a comprehensive testing algorithm for hepatitis B management. Ensure a separate vial is sent if ordered with additional HBV DNA quantification tests. Collection requires specific handling and centrifugation instructions as provided.
Limitations
Serum specimens from individuals taking biotin supplements at high doses may yield false-positive results due to interference with the assay. Anti-HBe test results should be considered in the context of the patient’s overall health and other laboratory findings. Extremely high titers of interfering antibodies can cause false-positive results, although test design minimizes these effects. The appearance of anti-HBe does not rule out chronic HBV infection. Performance characteristics have not been established for specimen types other than serum or when used with other manufacturers' assays.
FDA ApprovedNew York Approved
Methodology
Immunoassay (ECLIA)
Biomarkers
LOINC Codes
- 33463-1
- 33463-1
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.7 mL
Minimum Volume
0.5 mL
Container
Plastic vial
Collection Instructions
Centrifuge blood collection tube per manufacturer's instructions within 2 hours, aliquot serum into a plastic vial.
Patient Preparation
Patient should not take multivitamins or dietary supplements containing biotin (vitamin B7) 24 hours prior to collection.
Causes for Rejection
Gross hemolysis, lipemia, or icterus.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 hours |
| Refrigerated | 6 days |
| Frozen | 90 days |