Hepatitis B Virus Past Exposure Panel, Serum
Use
This test panel is utilized for screening for past exposure to hepatitis B virus (HBV) and determining HBV infection and immunity status. It is useful prior to initiating chemotherapy, immunosuppressive therapy, or organ transplantation, ensuring that patients are properly assessed for the presence of past or active hepatitis B viral infection. This helps in mitigating the risk of HBV reactivation which can occur in carriers under immunosuppressive conditions.
Special Instructions
The testing algorithm includes a reflex test for hepatitis B surface antigen (HBsAg) confirmation, which will be performed at an additional charge if the HBsAg test is reactive. The test uses serum collected in a serum gel tube with specific handling procedures to maintain sample integrity. Patients should avoid biotin-containing supplements for 24 hours before collection to prevent interference.
Limitations
The test performance characteristics are not established for grossly icteric, lipemic, or hemolyzed specimens, nor for those containing particulate matter or collected post-mortem. It's crucial to adhere to stable specimen conditions to avoid specimen rejection, and note that the test does not differ between vaccine-induced immunity and recovery from infection, as both may produce a positive anti-HBs result.
FDA ApprovedNew York Approved
Methodology
Immunoassay (ECLIA)
Biomarkers
LOINC Codes
- 77190-7
- 13952-7
- 5196-1
- 5193-8
- 10900-9
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
1.2 mL
Minimum Volume
0.9 mL
Container
Plastic vial
Collection Instructions
Centrifuge blood collection tube per manufacturer's instructions (eg, centrifuge and aliquot within 2 hours of collection for BD Vacutainer tubes). Aliquot serum into plastic vial.
Patient Preparation
For 24 hours before specimen collection, patient should not take multivitamins or dietary supplements containing biotin (vitamin B7).
Causes for Rejection
Gross hemolysis, gross lipemia, gross icterus
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 hours |
| Refrigerated | 6 days |
| Frozen | 90 days |