High-Performance Liquid Chromatography (HPLC) Hemoglobin Variant, Blood
Use
The test is useful for providing additional information that aids in the identification of hemoglobin variants. A large number of variants of hemoglobin have been recognized. Although many do not result in clinical or hematologic effects, clinical symptoms that can be associated with hemoglobin disorders include microcytosis, sickling disorders, hemolysis, erythrocytosis/polycythemia, cyanosis/hypoxia, and anemia (chronic, compensated, or episodic). For common and many uncommon hemoglobin variants, protein studies will be sufficient for definitive identification. High-performance liquid chromatography is a method that provides useful and supplementary information on most hemoglobin variants.
Special Instructions
Only orderable as part of a profile. Related profiles include Hemolytic Anemia Evaluation, Hemoglobin Electrophoresis Evaluation, Methemoglobinemia Evaluation, Erythrocytosis Evaluation, and Thalassemia and Hemoglobinopathy Evaluation.
Limitations
Some hemoglobin disorders and variants, including common alpha-thalassemia conditions, are not detected by screening methods and require further reflex testing for identification. Recent transfusion may mask protein results, including hemoglobin electrophoresis, hereditary persistence of fetal hemoglobin by flow cytometry, stability studies, and sickle solubility studies. Some variants can originate from donor blood products. Bone marrow transplant results may be atypical and should be interpreted in the context of clinical information. Certain therapies can cause artefactual effects in protein studies.
New York Approved
Methodology
Other
Biomarkers
Result Turnaround Time
1 day
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
Not provided
Minimum Volume
1 mL
Container
Whole Blood EDTA
Collection Instructions
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 10 days |