Histone H3 Trimethyl K27 Immunostain, Technical Component Only
Use
The Histone H3 Trimethyl K27 Immunostain is used for the diagnosis of malignant peripheral nerve sheath tumors and diffuse midline gliomas H3 K27M-mutant. Histone H3 trimethyl-K27 is a bivalent epigenetic regulator involved in gene silencing. Evaluation of its expression is particularly useful in diagnosing diffuse midline gliomas, which are more common in children and can also appear in adults, affecting regions like the pons, thalamus, and spinal cord. H3 K27M mutations are marked by global reduction of Histone H3 trimethyl-K27, aiding in distinguishing these tumors from other histologic mimics such as malignant peripheral nerve sheath tumors.
Special Instructions
Not provided.
Limitations
Interpretation must be performed by a qualified pathologist within the context of the patient's clinical history and other diagnostic tests. The test does not include pathologist interpretation. Paraffin sections are optimally cut within six weeks as age of sections can affect immunoreactivity. Unsuitable glass slides, due to surface chemistry alterations, can result in inconsistent staining.
FDA ApprovedNew York Approved
Methodology
Immunoassay (IHC)
Biomarkers
Histone H3 trimethyl-K27
Protein
LOINC Codes
- Order only;no result
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Storage Instructions
Maintain at ambient temperature when shipping is preferred. Refrigerated temperature is also acceptable.
Causes for Rejection
Wet/frozen tissue, cytology smears, nonformalin fixed tissue, nonparaffin embedded tissue, noncharged slides, ProbeOn slides, Snowcoat slides