HMB45 Immunostain, Technical Component Only
Use
The HMB45 immunostain is useful for the identification of activated and neoplastic melanocytes. This test identifies an antigen associated with a pre-melanosomal glycoprotein found in activated and neoplastic melanocytes. Most melanomas (approximately 90%) react with HMB45. HMB45 staining is cytoplasmic and is usually diffuse but may be focal. Benign nevi (moles) and other tumors that have melanin production such as peripheral nerve sheath tumors also stain positively.
Special Instructions
This test includes only the technical performance of the stain without pathologist interpretation. Technical component only stains should not be ordered with PATHC / Pathology Consultation. If ordered with PATHC, the technical component stains will be canceled. Attach the green 'Attention Pathology' address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Limitations
The test does not include pathologist interpretation, only technical performance of the stain. Interpretation is required to be performed in the context of the patient's clinical history and by a qualified pathologist. The age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen dependent. The charge of glass slides can be affected by environmental factors, potentially altering slide staining.
FDA ApprovedNew York Approved
Methodology
Immunoassay (IHC)
Biomarkers
LOINC Codes
- Order only;no result
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Storage Instructions
Preferred ambient, alternatively refrigerated.
Causes for Rejection
Wet/frozen tissue, Cytology smears, Nonformalin fixed tissue, Nonparaffin embedded tissue, Noncharged slides, ProbeOn slides, Snowcoat slides