Immunoglobulin Gene Rearrangement, Blood
Use
The Immunoglobulin Gene Rearrangement test is useful for determining whether a B-cell or plasma cell population is polyclonal or monoclonal using whole blood specimens. It helps in identifying neoplastic cells as having B-cell or plasma cell differentiation. Additionally, the test is used to monitor for a persistent neoplasm by detecting an immunoglobulin gene rearrangement profile similar to one from a previous neoplastic specimen. The test also aids in understanding the presence of a prominent immunoglobulin gene rearrangement profile in the context of neoplastic B-cell or plasma cell clones.
Special Instructions
Not provided.
Limitations
This test is not 100% sensitive or specific. False-negative results may occur if the immunoglobulin gene has numerous point alterations introduced during somatic hypermutation, preventing PCR primer binding. The assay may not detect clonal cells that have not rearranged the Ig genes evaluated or are below the sensitivity level of the assay (requires at least 1%-5% clonal presence). False-positive results, though rare, may appear if a prominent clone is sampled from polyclonal expansion. The test cannot determine the differentiation of clonal cells or if a prominent clone is physiological or neoplastic.
New York Approved
Methodology
PCR-based (PCR)
Biomarkers
No genes
Gene
LOINC Codes
- 61113-7 - Ig gene Rear Bld/T
- 34574-4 - Path report.final Dx Spec
- 19139-5 - Pathologist name
Result Turnaround Time
5-7 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
4 mL
Minimum Volume
1 mL
Container
Lavender top (EDTA); Yellow top (ACD acceptable)
Collection Instructions
Invert several times to mix blood. Send whole blood specimen in original tube. Do not aliquot.
Causes for Rejection
Gross hemolysis: Reject; Moderately to severely clotted: Reject
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 7 days |
| Refrigerated | 7 days |