Keratin 5 (KRT5) Immunostain, Technical Component Only
Use
Keratin 5 is a type II cytokeratin that dimerizes with the type I cytokeratin 14 forming intermediate filaments in the basal layer of the epidermis. It plays a role in differentiating mesothelioma and squamous cell carcinoma versus adenocarcinoma because it is usually positive in mesotheliomas and negative in adenocarcinomas. This makes it useful in separating mesotheliomas from pulmonary adenocarcinomas.
Special Instructions
Not provided.
Limitations
The test does not include pathologist interpretation, only technical performance of the stain. If interpretation is required, order PATHC / Pathology Consultation. Age of a cut paraffin section can affect immunoreactivity; best practice is for paraffin sections to be cut within 6 weeks. The charge of glass slides can be affected by environmental factors, altering slide staining. Sending unsuitable slides can result in inconsistent staining.
FDA ApprovedNew York Approved
Methodology
Immunoassay (IHC)
Biomarkers
Keratin 5
Protein
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Attach the green 'Attention Pathology' and pink Immunostain Technical Only labels to the transport container.
Storage Instructions
Minimize time slides are stored after unpackaging; limit exposure to humidity and heat.
Causes for Rejection
Wet/frozen tissue, cytology smears, non-formalin fixed or paraffin embedded tissue, noncharged slides, ProbeOn or Snowcoat slides.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |