Kingella kingae, Molecular Detection, PCR, Varies
Use
Aiding in the diagnosis of Kingella kingae infection using tissue or synovial fluid specimens. Kingella kingae is a fastidious short gram-negative bacillus that may colonize the oropharynx of young children. It can lead to invasive disease, often causing bone and joint infections, particularly in children aged 6 to 36 months. The organism may also cause endocarditis in patients of any age. This PCR test helps in diagnosing Kingella kingae infection, which is challenging due to the organism's fastidious nature in culture.
Special Instructions
The test requires the specimen to be processed in an environment that minimizes contamination by Kingella kingae DNA. If not ordering electronically, complete, print, and send a Microbiology Test Request form with the specimen.
Limitations
A negative result does not negate the presence of the organism or active disease due to possible PCR inhibition, sequence variability, or low levels of Kingella kingae DNA. The test does not detect species of Kingella other than kingae or negevensis and may show cross-reactivity with Kingella negevensis.
New York Approved
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 65809-6
- 31208-2
- 65809-6
Result Turnaround Time
2-7 days
Related Documents
For more information, please review the documents below
Specimen
Other
Volume
0.5 mL
Minimum Volume
Not provided
Container
Lavender top (EDTA) preferred, alternatives include Pink top (EDTA), royal blue top (EDTA), sterile vial with EDTA-derived aliquot, red clot tube (no anticoagulant), or sterile container.
Collection Instructions
Send specimen in original tube (preferred).
Storage Instructions
Refrigerated (preferred) <7 days / Frozen <7 days
Causes for Rejection
Tissue in formalin, formaldehyde, or acetone, decalcified bone, bone marrow, slides
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient |
| Refrigerated | <7 days |
| Frozen | <7 days |