Liver/Kidney Microsome Type 1 Antibodies, Serum
Use
The Liver/Kidney Microsome Type 1 Antibodies (anti-LKM-1) test is significant for the evaluation of autoimmune hepatitis type 2, particularly in patients exhibiting clinical symptoms of liver disease with unknown etiology. This test assists in the differential diagnosis of autoimmune liver disease by detecting anti-LKM-1 antibodies which are commonly associated with autoimmune hepatitis type 2. These antibodies target the cytochrome P450 2D6 (CYP2D6) enzyme, aiding in distinguishing AIH-2 from other liver conditions. It is crucial for determining an appropriate treatment regime, especially important in children and young adults where AIH-2 is more prevalent and aggressive.
Special Instructions
This test requires completion of a Gastroenterology and Hepatology Test Request form if not ordering electronically. It is important that the specimen be centrifuged and serum aliquoted into a plastic vial. Stability conditions must be strictly followed to ensure sample integrity.
Limitations
The presence of anti-LKM-1 antibodies should not be solely relied upon for a diagnosis of autoimmune hepatitis (AIH) or prediction of disease development. These antibodies must be considered in conjunction with other serological findings such as antinuclear antibodies, smooth muscle antibodies, and filamentous-actin antibodies to avoid diagnostic errors, particularly in cases where hepatitis C might produce similar antibody responses. Given that the epitopes recognized by the anti-LKM-1 in HCV are different, clinical correlation is required.
FDA ApprovedNew York Approved
Methodology
Immunoassay (ELISA)
Biomarkers
LOINC Codes
- 32220-6
- 32220-6
Result Turnaround Time
2-4 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.5 mL
Minimum Volume
0.4 mL
Container
Plastic vial
Collection Instructions
Centrifuge and aliquot serum into a plastic vial.
Storage Instructions
Refrigerated (preferred) for up to 21 days or 21 days if frozen.
Causes for Rejection
Gross hemolysis, Gross lipemia, Heat treated
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 21 days |
| Frozen | 21 days |