Low-Grade Fibromyxoid Sarcoma (LGFMS), 16p11.2 (FUS or TLS) Rearrangement, FISH, Tissue
Use
This test is useful for supporting the diagnosis of low-grade fibromyxoid sarcoma (LGFMS) when used in conjunction with an anatomic pathology consultation. LGFMS is a rare malignant soft tissue tumor characterized by a bland fibroblastic spindle cell proliferation arranged in alternating fibrous and myxoid areas. These tumors often have the chromosome translocation t(7;16)(q33-34;p11) resulting in the fusion of FUS (also called TLS) on chromosome 16 to CREB3L2 on chromosome 7. In rare cases, a variant t(11;16)(p11;p11) has been described, with FUS fused to CREB3L1 (OASIS).
Special Instructions
Not provided.
Limitations
This test is not approved by the US Food and Drug Administration and is best used as an adjunct to existing clinical and pathologic information. Fixatives other than formalin may not be successful for FISH assays, although nonformalin-fixed samples will not be rejected. Paraffin-embedded tissues that have been decalcified are typically unsuccessful for FISH analysis.
New York Approved
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
No genes
Gene
LOINC Codes
- 101383-8 - FUS gene rearrangements Bld/T FISH
- 50397-9 - Mol dx interp Bld/T Ql
- 69965-2 - Clinical cytogeneticist review
- 62356-1 - Chrom analy result (ISCN)
- 42349-1
- 31208-2 - Specimen source
- 80398-1 - Unique ID Current sample
- 85069-3 - Lab test method
- 48767-8 - Annotation comment Imp
- 18771-6 - Provider signing name
- 62364-5 - Test performance info Spec
Result Turnaround Time
7-10 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Submit a formalin-fixed, paraffin-embedded (FFPE) tumor tissue block. Blocks prepared with alternative fixation methods may be acceptable; provide fixation method used.
Causes for Rejection
All specimens will be evaluated by Mayo Clinic Laboratories for test suitability.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Preferred |