MayoComplete Targeted RNA Sequencing Panel, Next-Generation Sequencing, Tumor
Use
This test is primarily used for identifying gene fusions that aid in diagnosing solid tumors and secondarily for identifying fusions with therapeutic or prognostic significance. It evaluates 1445 genes for somatic gene fusions and specific known abnormal variants in MET, EGFR, and BCOR genes, without assessing germline alterations. Molecular analysis of biomarkers is utilized in oncology to support diagnosis, prognosis, and therapeutic management of patients.
Special Instructions
Not provided.
Limitations
This assay is not validated for the detection of single nucleotide variations, insertions, deletions, copy number alterations, or gene expression. It primarily detects in-frame gene fusions and certain known transcript variants. Rare variants may lead to false-negative or false-positive results. The sensitivity depends on several factors, including tumor percentage and expression level. A negative result does not completely rule out the presence of fusions or variants that may be below the detection limits. It's validated on cytology slides and FFPE tissues; improper specimen handling can affect results.
New York Approved
Methodology
NGS (Targeted)
Biomarkers
MET, EGFR, BCOR
Gene
LOINC Codes
- 95123-6 - Gene fusion details Bld/T
- 82939-0 - Genetic variant details Bld/T
- 69047-9 - Geneticist review
- 48767-8 - Annotation comment Imp
- 31208-2 - Specimen source
- 80398-1 - Unique ID Current sample
- 85069-3 - Lab test method
- 62364-5 - Test performance info Spec
- 18771-6 - Provider signing name
Result Turnaround Time
12-20 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Formalin-fixed, paraffin-embedded tissue block
Collection Instructions
Submit a tissue block fixed in 10% neutral buffered formalin with adequate tumor nuclei.
Causes for Rejection
Decalcified specimens are not acceptable.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |