Mumps Virus, Molecular Detection, PCR, Buccal
Use
Rapid qualitative detection of mumps virus using buccal swab specimens. The mumps virus is a single-stranded, negative-sense RNA paramyxovirus belonging to the Rubulavirus family. Laboratory diagnosis can be conducted through serologic detection of mumps-specific IgM antibodies, molecular detection of mumps virus RNA, or viral culture. The real-time PCR method offers quick laboratory confirmation after symptom onset compared to serologic testing and provides faster results than viral culture. Buccal swabs are preferred for detection, but urine may also be collected.
Special Instructions
Recommended as a first-line test if symptoms suggest mumps. If IgM antibodies against mumps are detected previously, this test confirms infection. Specimens should be transported immediately. Perform collection by swabbing around the buccal cavity to maximize cell recovery. Swab must be placed into viral transport media.
Limitations
A negative test does not rule out mumps virus infection and should be considered alongside clinical findings and serologic results to avoid false negatives due to improper sample collection or viral variants. No cross-reactivity with other bacterial, fungal, or viral organisms causing similar disease.
New York Approved
Methodology
PCR-based (RT-PCR)
Biomarkers
LOINC Codes
- 47532-7
- 47532-7
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Buccal Swab
Volume
Entire collection
Minimum Volume
0.3 mL
Container
Sterile container with transport media
Collection Instructions
Collect specimen by swabbing back and forth over mucosal surface around buccal cavity to maximize recovery of cells. Swab must be placed into viral transport media (M4-RT, M4, M5, Barthels FlexTrans Media or Jiangsu Transport Media).
Storage Instructions
Refrigerated (preferred): 7 days. Frozen: 7 days.
Causes for Rejection
Throat swab, E-swab, calcium alginate-tipped swab, wood swab, dry swab, or transport swab containing gel or charcoal additive
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 7 days |