MYB (6q23) Rearrangement FISH, Tissue
Use
Identifying MYB gene rearrangements is crucial for supporting the diagnosis of primary salivary gland adenoid cystic carcinomas, especially when used in conjunction with an anatomic pathology consultation. Adenoid cystic carcinoma (ACC) is aggressive and distinguished from other salivary gland tumors by its clinical behavior. Translocations involving MYB and NFIB genes are common in primary salivary gland ACC but not in other salivary tumors, thus confirming ACC through MYB break-apart probes in fluorescence in situ hybridization is diagnostic.
Special Instructions
A pathology report is mandatory for testing to proceed—this includes drafts or preliminary surgical pathology reports. The following must be included: patient name, block number, date of collection, tissue source, and reason for testing. Consultative diagnostic work should be directed to the pathologist's discretion, and any additional probes utilized will have results included in the final report.
Limitations
This test has not been approved by the FDA and serves as a laboratory-developed assay to complement clinical and pathologic data. Non-formalin fixatives may compromise results, and paraffin-embedded decalcified bones have a success rate of about 50% in FISH analysis. The absence of MYB rearrangements doesn't exclude ACC as some cases lack these translocations.
New York Approved
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
LOINC Codes
- 50397-9
- 69965-2
- 62356-1
- 42349-1
- 31208-2
- 80398-1
- 85069-3
- 18771-6
- 48767-8
- 62364-5
Result Turnaround Time
7-10 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Tissue block or slides
Collection Instructions
Submit a formalin-fixed, paraffin-embedded tumor tissue block. For tissue slides: submit 2 consecutive unstained, positively charged, unbaked slides and 1 slide stained with hematoxylin and eosin.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |