Mycobacterium tuberculosis Complex Species Identification, PCR, Varies
Use
This assay provides a species-level identification of microbiologic culture isolates previously identified to be a member of the Mycobacterium tuberculosis complex. Species-level identification can be important for patient care or for epidemiologic investigations. For example, the species-level identification of Mycobacterium bovis bacillus Calmette-Guerin (BCG) can assist with identification of disseminated infections following use of the vaccine as an adjuvant during chemotherapy.
Special Instructions
Not provided.
Limitations
Only isolates of Mycobacterium tuberculosis complex should be submitted and must be in pure culture. Nontuberculous mycobacteria should not be submitted. Mixed cultures will result in a delay because the M tuberculosis complex organism must be isolated before performing the PCR assay. This assay is not verified for direct detection from clinical specimens and is intended for use on microbiologic culture isolates identified as M tuberculosis complex.
FDA ApprovedNew York Approved
Methodology
PCR-based (Real-Time PCR)
Biomarkers
LOINC Codes
- 94576-6 - MTB complex sp Islt NAA+probe
- 94576-6 - MTB complex sp Islt NAA+probe
Result Turnaround Time
7-14 days
Related Documents
For more information, please review the documents below
Specimen
Other
Volume
Isolate with visible growth on solid media; if broth is sent, 3 mL or more of broth culture
Minimum Volume
Not provided
Container
Growth on solid slant media, eg, Middlebrook 7H10, 7H11 and Lowenstein Jensen; growth in broth medium, eg, Mycobacteria Growth Indicator Tube, 7H9 broth BACT/ALERT MP or VersaTREK
Collection Instructions
1. Bacterial organism must be submitted in pure culture, actively growing. Do not submit mixed cultures. 2. Place specimen in a large infectious container and label as an etiologic agent/infectious substance.
Causes for Rejection
Agar plate; Mixed culture