Neurofilament (2F11) Immunostain, Technical Component Only
Use
This test is useful for aiding in the identification of neoplastic cells of neural origin or those exhibiting neuronal differentiation. Neurofilament (NF) constitutes the main structural elements of neuronal axons and dendrites. NF subunits are present in neurons, neuronal processes, peripheral nerves, and sympathetic ganglion cells. In brain parenchyma, NF labels the axons of the central nervous system. Within tumors, only neoplastic cells of neural origin or those exhibiting neuronal differentiation have been observed to express NF. Positive immunostaining has been observed in neuromas, gangliogliomas, neuroblastomas, and medulloblastomas. Other tumors that can stain for NF include pheochromocytoma, chemodectomas, and carcinoid tumors.
Special Instructions
Not provided.
Limitations
The test does not include pathologist interpretation, it only provides technical performance of the stain. Age of cut paraffin sections can affect immunoreactivity, as stability thresholds vary and are antigen-dependent. It is suggested that paraffin sections be cut within 6 weeks. Environmental factors can also affect the charge of glass slides, altering staining results. Unsuitable glass slides may lead to inconsistencies due to poor surface chemistry.
FDA ApprovedNew York Approved
Methodology
Immunoassay (IHC)
Biomarkers
Neurofilament
Protein
LOINC Codes
- Order only; no result
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Preferred: Formalin-fixed, paraffin-embedded tissue block or 2 unstained, positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.
Storage Instructions
Attach labels to the transport container as instructed.
Causes for Rejection
Wet/frozen tissue, cytology smears, nonformalin fixed tissue, nonparaffin embedded tissue, noncharged slides, ProbeOn slides, Snowcoat slides