OCT3/4 Immunostain, Technical Component Only
Use
Octamer-binding transcription factor 3/4 (OCT4), also known as POU5F1, is a transcription factor expressed by embryonal stem cells and germ cells. Staining for OCT4 can aid in the diagnosis of testicular or ovarian germ cell tumors; it is highly specific for germ cell neoplasia, especially seminomas. This test is useful for the identification of germ cell tumors.
Special Instructions
Not provided.
Limitations
The test does not include pathologist interpretation, only technical performance of the stain. Age of a cut paraffin section can affect immunoreactivity, as stability thresholds vary widely among published literature and are antigen dependent. Environmental factors can affect the charge of glass slides and alter slide staining, which may lead to inconsistent results due to poor slide surface chemistry. Best practices involve minimizing time slides are stored after being unpackaged and limiting exposure to high humidity and heat.
FDA ApprovedNew York Approved
Methodology
Immunoassay (IHC)
Biomarkers
OCT3/4
Protein
LOINC Codes
- Order only
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Causes for Rejection
Wet/frozen tissue, cytology smears, nonformalin fixed tissue, nonparaffin embedded tissue, noncharged slides, ProbeOn slides, Snowcoat slides