Oligoclonal Banding, Serum and Spinal Fluid
Use
This test is useful for the diagnosis of multiple sclerosis, particularly in patients with equivocal clinical presentation and radiological findings. By detecting increased intrathecal immunoglobulin synthesis, it helps in identifying MS. The oligoclonal band assay detects 2 or more unique IgG bands in the cerebrospinal fluid, indicating a positive result for MS. However, the presence of CSF-specific IgG synthesis is not exclusive to MS and may occur in other inflammatory CSF diseases.
Special Instructions
Both serum and spinal fluid samples are required for this test. Spinal fluid must be collected within 7 days of serum collection. Ensure the serum is centrifuged and aliquoted into a plastic vial within 2 hours of collection. Label specimens appropriately as serum and spinal fluid.
Limitations
The test is not specific for multiple sclerosis, as increased intrathecal immunoglobulin synthesis can also occur in other neurologic diseases including infectious, inflammatory, cerebrovascular, and paraneoplastic disorders. Clinical correlation is recommended. Additionally, grossly hemolyzed, lipemic, or icteric samples are acceptable for testing, but standard collection and storage instructions must be followed to preserve sample integrity.
FDA ApprovedNew York Approved
Methodology
Immunoassay (Isoelectric Focusing)
Biomarkers
LOINC Codes
- 100757-4
- 49852-7
- 100756-6
- 100755-8
Result Turnaround Time
4-7 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.5 mL
Minimum Volume
0.4 mL
Container
Plastic vial
Collection Instructions
Within 2 hours of collection, centrifuge and aliquot serum into a plastic vial. Label specimen as serum.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 14 days |
| Refrigerated | 14 days |
| Frozen | 14 days |