Use

This test is useful for evaluating suspected hereditary spherocytosis-associated hemolytic anemia and confirming or detecting mild spherocytosis. Spherocytes are osmotically fragile cells that rupture more easily in a hypotonic solution than normal red blood cells due to their low surface area to volume ratio. The test measures the degree of hemolysis in spherocytes, which is typically more pronounced in hereditary spherocytosis than other causes of spherocytosis.