Use

This test is useful for screening and confirming the diagnosis of paroxysmal nocturnal hemoglobinuria (PNH) and for monitoring patients with PNH. PNH is an acquired hematologic disorder characterized by nocturnal hemoglobinuria, chronic hemolytic anemia, thrombosis, pancytopenia, and acute or chronic myeloid malignancies in some patients. It is a stem cell disorder affecting various blood cell lines, resulting in a deficiency of GPI-linked proteins. Variants in the PIGA gene are commonly identified in PNH patients, confirming the biological defect. Flow cytometry assays detect GPI-linked protein absence in blood cells, providing a more reliable diagnosis by avoiding the issues of RBC-based diagnostic methods.