Phosphoglycerate Kinase Enzyme Activity, Blood
Use
The Phosphoglycerate Kinase Enzyme Activity test is useful for the evaluation of individuals with Coombs-negative nonspherocytic hemolytic anemia, especially if there is a pattern of X-linked inheritance. It is also important for patients with myopathic or neurologic symptoms as it helps in diagnosing phosphoglycerate kinase deficiency, an X-linked disorder. This enzyme plays a critical role in glycolysis by converting 1,3-diphosphoglycerate to 3-phosphoglyceric acid, a step that generates adenosine triphosphate (ATP).
Special Instructions
Not provided.
Limitations
Phosphoglycerate kinase deficiency may not always correlate with the enzyme activity levels. Female heterozygous individuals may show mild symptoms. Recent transfusions or reticulocytosis may affect the enzyme activity measurements. It's important to compare the activities of other red blood cell enzymes to avoid diagnostic errors.
New York Approved
Methodology
Automated Analyzer (Clinical Chemistry)
Biomarkers
Phosphoglycerate Kinase
Analyte
LOINC Codes
- 44053-7 - PGK RBC-cCnt
- 44053-7 - PGK RBC-cCnt
Result Turnaround Time
1-6 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
6 mL
Minimum Volume
1 mL
Container
Preferred: Yellow top (ACD solution B), Acceptable: Lavender top (EDTA) or Yellow top (ACD solution A)
Collection Instructions
Invert several times to mix blood and send whole blood specimen in original tube. Do not aliquot.
Storage Instructions
Refrigerated for up to 20 days.
Causes for Rejection
Gross hemolysis, Fully clotted
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 20 days |