Red Blood Cell (RBC) Enzyme Evaluation, Blood
Use
This test is useful for identifying defects in red blood cell enzyme metabolism and evaluating patients with Coombs-negative hemolytic anemia. Erythrocyte enzyme deficiencies are inherited causes of hemolytic anemia, with some being common, such as glucose 6-phosphate dehydrogenase (G6PD) deficiency, and others rare, affecting only a few families globally. Testing is crucial for a consultative evaluation to explore red cell enzyme activity as a cause of early red cell destruction.
Special Instructions
The test involves a consultative evaluation at Mayo Clinic Laboratories. For a more complete interpretation, it is strongly recommended to include Metabolic Hematology Patient Information, which should contain clinical history.
Limitations
Recent transfusion can mask a patient's intrinsic enzyme activity, leading to unreliable results. High white blood cell counts may interfere by falsely raising enzyme activity. Some enzyme deficiencies can be masked by reticulocytosis, and comparing activities of other red blood cell enzymes in the panel can provide additional insights.
New York Approved
Methodology
Automated Analyzer (Clinical Chemistry)
Biomarkers
LOINC Codes
- 72695-0
- 2902-5
- 44051-1
- 59466-3
- 18771-6
- 2383-8
- 44054-5
- 32552-2
- 44053-7
- 72664-6
- 49216-5
- 44050-3
- 32546-4
Result Turnaround Time
2-13 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
12 mL
Minimum Volume
5 mL
Container
Yellow top (ACD solution B)
Collection Instructions
Send whole blood specimen in original tube. Do not aliquot.
Causes for Rejection
Gross hemolysis
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 11 days |