Respiratory Syncytial Virus (RSV) In Situ Hybridization, Technical Component Only
Use
This test aids in the identification of respiratory syncytial viral infection, specifically targeting subgroups RSV-A and RSV-B. The virus primarily affects young children by infecting alveolar pneumocytes, creating a characteristic syncytial multinucleated cell structure. The test is designed to specifically identify RSV-A and RSV-B without cross-reactions with other common viruses in formalin-fixed, paraffin-embedded tissue samples.
Special Instructions
This test includes only the technical performance of the stain without a pathologist interpretation. Clients should not order this test with PATHC / Pathology Consultation as the technical stains will be canceled if ordered together. For complete diagnostic or second opinion cases, PATHC should be ordered separately.
Limitations
The test does not provide pathologist interpretation and should be used as a part of a broader diagnostic process. The quality of results may be affected by specimen integrity, especially if paraffin sections are older than 6 weeks, as age can impact immunoreactivity. Additionally, unsuitable glass slides can alter staining results, suggesting that slides should be used quickly after unpackaging and stored appropriately to prevent inconsistent results.
New York Approved
Methodology
Chromosomal / Cytogenetics (ISH)
Biomarkers
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Attach the green 'Attention Pathology' address label (T498) and the pink Immunostain Technical Only label to the outside of the transport container. Preferred specimens include formalin-fixed, paraffin-embedded blocks or 4 unstained glass slides cut at 4-micron thickness.
Storage Instructions
Store slides in conditions that minimize exposure to high humidity and heat to preserve slide quality.
Causes for Rejection
Wet/frozen tissue, cytology smears, nonformalin fixed tissue, nonparaffin embedded tissue, noncharged slides, ProbeOn slides, Snowcoat slides.