RUNX1::RUNX1T1 Translocation (8;21), Minimal Residual Disease Monitoring, Quantitative, Varies
Use
Detection of RUNX1::RUNX1T1 gene fusion in acute myeloid leukemia patients at the time of diagnosis. Minimal residual disease monitoring during the clinical and therapeutic course of these patients. RUNX1::RUNX1T1 minimal residual disease (MRD) monitoring in patients with acute myeloid leukemia (AML) with translocation t(8;21)(q22;q22) is useful for evaluating disease response after therapy and identifying individuals with increased risk of relapse.
Special Instructions
Necessary information required includes pertinent clinical history, date of collection, and specimen source (blood or bone marrow). Refrigerated specimens must arrive within 5 days of collection, and ambient specimens must arrive within 3 days of collection.
Limitations
The limit of detection for this test is 0.01%. Monitoring should be performed using the same method and laboratory for each subsequent specimen. Interassay variability can occur; result changes should not be considered significant if two single measurements differ by less than 0.5 log. More critical results, such as a change in the status of positivity or a 1 log or greater increase between two positive samples, should be repeated on a separate specimen with appropriate time interval to verify the result.
New York Approved
Methodology
PCR-based (RT-qPCR)
Biomarkers
LOINC Codes
- 72207-4
- 31208-2
- 69047-9
- 18771-6
Result Turnaround Time
4-8 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
10 mL
Minimum Volume
8 mL
Container
Lavender top (EDTA) or yellow top (ACD-B)
Collection Instructions
Invert several times to mix blood. Send whole blood specimen in original tube. Do not aliquot. Label specimen as blood.
Causes for Rejection
Gross hemolysis, Bone marrow core biopsies, Heparin sample, Paraffin-embedded bone marrow clots, Slides, Paraffin shavings, Moderately to severely clotted.
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 5 days |