Shigella Culture, Feces
Use
This test is used to determine whether Shigella species may be the cause of diarrhea. It provides evidence of the presence of Shigella bacteria in feces in a viable state, allowing for isolation and susceptibility testing. Reflexive testing for Shigella species from nucleic acid amplification test-positive stool is included. However, it is generally not useful for patients hospitalized more than 3 days due to low yield and likelihood of identifying new pathogens.
Special Instructions
Minnesota healthcare professionals must report confirmed or suspected cases of shigellosis to the Minnesota Department of Health. Clients should refer to local health departments regarding public health submission of Shigella isolates. Specimen must arrive within 96 hours of collection. Specimen source is required.
Limitations
The yield of Shigella species is reduced when specimens are delayed in transit to the laboratory, especially if more than 2 hours from collection for unpreserved specimens. This test is not useful for patients hospitalized for more than 3 days. Local public health requirements may mandate submission of isolates to public health laboratories.
FDA ApprovedNew York Approved
Methodology
Culture-based
Biomarkers
LOINC Codes
- 17576-0
- 88586-3
Result Turnaround Time
3-5 days
Related Documents
For more information, please review the documents below
Specimen
Stool
Volume
Representative portion of fecal specimen
Minimum Volume
1 mL
Container
Commercially available transport system specific for recovery of enteric pathogens from fecal specimens
Collection Instructions
Collect fresh feces and submit 1 gram or 5 mL in container with transport medium. Place feces in preservative within 2 hours of collection. Place vial in a sealed plastic bag.
Patient Preparation
The patient should not use antacids, barium, bismuth, antidiarrheal medication, or oily laxatives before specimen collection.
Causes for Rejection
Unpreserved feces, ECOFIX preservative, Formalin or PVA fixative
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 4 days |
| Refrigerated | 4 days |