SV40 Immunostain, Technical Component Only
Use
Simian virus 40 (SV40) polyomavirus immunohistochemistry can be used in the diagnosis of polyomavirus-associated diseases, such as BK virus-associated nephropathy and JC virus-associated progressive multifocal leukoencephalopathy. The SV40 assay does not identify the specific polyomavirus detected, as BK and JC viruses show approximately 70% similarity to SV40. This test has been shown to not crossreact with Merkel cell polyomavirus.
Special Instructions
This test includes only technical performance of the stain; no pathologist interpretation is provided. Technical component only stains should not be ordered with PATHC / Pathology Consultation. If ordered with PATHC, the technical component stains will be canceled. Any immunohistochemistry (IHC)/in situ hybridization (ISH) stain performed as a part of the PATHC will be performed at the reviewing pathologist's discretion at an additional charge.
Limitations
This test does not include pathologist interpretation, only technical performance of the stain. If interpretation is required, order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. The age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen dependent. Best practice is for paraffin sections to be cut within 6 weeks.
New York Approved
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Attach the green 'Attention Pathology' address label (T498) and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Storage Instructions
Best practices for storage of positively charged slides: Minimize time slides are stored after being unpackaged. Limit exposure to high humidity and heat. Minimize exposure to plastics.
Causes for Rejection
Wet/frozen tissue, Cytology smears, Nonformalin fixed tissue, Nonparaffin embedded tissue, Noncharged slides, ProbeOn slides, Snowcoat slides
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Ambient (preferred) |
| Refrigerated | Refrigerated |