T-Cell Intracellular Antigen 1 (TIA-1) Immunostain, Bone Marrow, Technical Component Only
Use
The T-Cell Intracellular Antigen 1 (TIA-1) Immunostain test is useful for characterizing neoplasms of cytotoxic T cells or natural killer cells. TIA-1 shows a granular cytoplasmic staining pattern due to its presence in cytotoxic granules, involved in cytotoxic cell-mediated immune responses. It is expressed in normal cytotoxic T cells, natural killer (NK) cells, and granulocytes, providing valuable insight into immune-related disorders.
Special Instructions
Not provided.
Limitations
This test is optimized for use on B5-fixed, decalcified, paraffin-embedded bone marrow specimens. The age of cut paraffin sections can significantly impact immunoreactivity, with stability varying widely based on the antigen. Environmental factors may affect the charge of glass slides, altering slide staining and possibly causing inconsistent results. Best practices for storage include minimizing time slides are unpackaged and limiting exposure to humidity, heat, and plastics.
FDA ApprovedNew York Approved
Methodology
Immunoassay (IHC)
Biomarkers
TIA-1
Protein
Result Turnaround Time
1-3 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Immunostain Technical Only Envelope
Collection Instructions
Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container. Preferred: Formalin-fixed, paraffin-embedded tissue block or 2 unstained, positively charged glass slides per test ordered; sections 4-microns thick.
Storage Instructions
Ambient (preferred); Refrigerated also acceptable.
Causes for Rejection
Wet/frozen tissue, cytology smears, nonformalin-fixed tissue, nonparaffin embedded tissue, noncharged slides, ProbeOn slides, Snowcoat slides