T-Cell Receptor V-Beta Repertoire Analysis, Spectratyping, Blood
Use
This test is used for the assessment of T-cell receptor diversity in various clinical contexts, including inborn errors of immunity (formerly primary immunodeficiencies), monitoring immune reconstitution post-hematopoietic stem cell transplantation, and assessing repertoire changes in autoimmune diseases and viral infections. It provides a global view of TCR repertoire diversity, which is useful for most clinical applications where this level of assessment is required.
Special Instructions
Not provided.
Limitations
This is a qualitative/semiquantitative assay and does not quantify the amount of transcript for each T-cell receptor. It is not a deep sequencing assay and doesn't provide the granularity of information offered by deep sequencing. Not validated for determining clonality in hematologic malignancies. Should not be ordered for low CD3+ T-cell count (<70 cells/mcL) or without prior discussion for patients over 40.
New York Approved
Methodology
PCR-based (PCR)
Biomarkers
TCR V-beta
Other
LOINC Codes
- 21751-3 - TCRB gene Rear Bld/T Ql
- 8122-4 - CD3 Cells # Bld
- 24467-3 - CD3+CD4+ Cells # Bld
- 14135-8 - CD3+CD8+ Cells # Bld
- 82501-8 - TRBV2 seg/TRBV seg total NFr Bld
- 82500-0 - TRBV3-1 seg/TRBV seg total NFr Bld
- 82499-5 - TRBV4 seg/TRBV seg total NFr Bld
- 82498-7 - TRBV5 seg/TRBV seg total NFr Bld
- 82497-9 - TRBV6 seg/TRBV seg total NFr Bld
- 82496-1 - TRBV7 seg/TRBV seg total NFr Bld
- 82495-3 - TRBV9 seg/TRBV seg total NFr Bld
- 82494-6 - TRBV10 seg/TRBV seg total NFr Bld
- 82493-8 - TRBV11 seg/TRBV seg total NFr Bld
- 82492-0 - TRBV12 seg/TRBV seg total NFr Bld
- 82491-2 - TRBV13 seg/TRBV seg total NFr Bld
- 82490-4 - TRBV14 seg/TRBV seg total NFr Bld
- 82489-6 - TRBV15 seg/TRBV seg total NFr Bld
- 82488-8 - TRBV16 seg/TRBV seg total NFr Bld
- 82487-0 - TRBV18 seg/TRBV seg total NFr Bld
- 82486-2 - TRBV19 seg/TRBV seg total NFr Bld
- 82485-4 - TRBV20-1 seg/TRBV seg total NFr Bld
- 82484-7 - TRBV24-1 seg/TRBV seg total NFr Bld
- 82483-9 - TRBV25 seg/TRBV seg total NFr Bld
- 82482-1 - TRBV27 seg/TRBV seg total NFr Bld
- 82481-3 - TRBV28 seg/TRBV seg total NFr Bld
- 82480-5 - TRBV29 seg/TRBV seg total NFr Bld
- 82479-7 - TRBV30 seg/TRBV seg total NFr Bld
- 69047-9 - Geneticist review
- 48767-8 - Annotation comment Imp
- 85069-3 - Lab test method
- 62364-5 - Test performance info Spec
- 18771-6 - Provider signing name
Result Turnaround Time
20-24 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
10 mL
Minimum Volume
5 mL
Container
Lavender top (EDTA)
Collection Instructions
Send whole blood specimen in original tube. Do not aliquot.
Storage Instructions
Specimens must be received on weekdays and by 4 p.m. on Friday. Arrive within 24 hours of collection.
Causes for Rejection
Gross hemolysis, clotted specimens
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 48 hours |