T-Cell Subsets, Regulatory (Tregs), Blood
Use
The T-Cell Subsets, Regulatory (Tregs), Blood test is crucial for evaluating patients with clinical features of IPEX (immune dysregulation, polyendocrinopathy, enteropathy, X-linked inheritance) and other primary immunodeficiencies. It is also useful in autoimmune diseases, allergy and asthma, and monitoring graft-vs-host disease post-hematopoietic stem cell transplantation. This test helps in understanding the regulatory T cells (Tregs) role in suppressing aberrant immune responses.
Special Instructions
Not provided.
Limitations
This test provides only quantitative information on various regulatory T cell (Treg) subsets and does not provide functional information about these populations. Molecular testing is needed to confirm a diagnosis of IPEX. The timing and consistency of blood collection are critical for monitoring lymphocyte subsets serially.
Methodology
Cell-based / Cytometry (Flow Cytometry)
Biomarkers
LOINC Codes
- 90413-6 - T-cell regulatory subsets Pnl Bld
- 89315-6 - CD4+CD25+CD45RA+CD127Low+ cells # Bld
- 89316-4 - CD4+CD25+CD45RO+CD127Low+ cells # Bld
- 26982-9 - CD4+CD25+ Cells # Bld
- 89319-8 - CD25+CD45RA+CD127L+/CD4 NFr Bld
- 89320-6 - CD25+CD45RO+CD127L+/CD4 NFr Bld
- 13332-2 - CD4+CD25+ Cells NFr Bld
- 89318-0 - CD25-CD127+ cells/CD4 Cells NFr Bld
- 89317-2 - CD4+CD25-CD127+ cells # Bld
- 69052-9 - Flow cytometry specialist review
- 24467-3 - CD3+CD4+ Cells # Bld
Result Turnaround Time
3-4 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
3 mL
Minimum Volume
1 mL
Container
Lavender top (EDTA)
Collection Instructions
Send whole blood specimen in original tube. Do not aliquot.
Causes for Rejection
Gross hemolysis, gross lipemia
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 72 hours |