Vitamin B5 (Pantothenic Acid) Bioassay
Use
The Vitamin B5 (Pantothenic Acid) Bioassay is used to determine the level of pantothenic acid in serum, which is essential for various physiological functions. Pantothenic acid, also known as Vitamin B5, is crucial for the biosynthesis of coenzyme A, which is involved in fatty acid metabolism, energy production, and the synthesis of various other compounds necessary for cellular processes. Deficiency in pantothenic acid can lead to fatigue, insomnia, depression, irritability, vomiting, stomach pains, burning feet, and upper respiratory infections.
Special Instructions
This test is performed on serum collected in a serum gel tube or a red top tube before transferring to a plastic vial for submission. It is crucial for the specimen to be frozen as soon as possible after collection to ensure stability and accuracy of results.
Limitations
The assay's performance characteristics are limited by its validation through BioAgilytix Diagnostics, and it is not approved or cleared by the US FDA. The assay is sensitive to conditions like gross hemolysis, lipemia, or contamination, which can cause rejection of the specimen. The test may not be readily orderable for clients in New York State due to lack of approval there.
Methodology
Microbiological Assay
Biomarkers
LOINC Codes
- 2722-7
- 2722-7
Result Turnaround Time
4-18 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.5 mL
Minimum Volume
0.2 mL
Container
Plastic vial
Collection Instructions
Allow specimen to clot for 30 minutes at room temperature. Centrifuge for 15 minutes at 1000 x g. Aliquot 0.5 mL serum into a plastic vial. Send frozen.
Causes for Rejection
Gross hemolysis, Gross lipemia, Specimens containing particulate matter or microbial contamination
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 7 days |
| Frozen | 21 days |