Zucchini (Cucurbita spp) IgE
Use
The detection and measurement of zucchini (Cucurbita spp) IgE antibodies are used to assist in the diagnosis of allergic reactions to zucchini as well as monitoring allergic load in sensitized patients. Allergic reactions to foods like zucchini can cause various symptoms including skin reactions, gastrointestinal discomfort, and anaphylaxis in severe cases. This test helps provide targeted IgE levels to support clinical assessments and management of food allergies related to zucchini.
Special Instructions
Collect serum using a red top or serum gel tube. Centrifuge and aliquot 0.5 mL of serum into a plastic vial for submission. Store and send samples refrigerated.
Limitations
The use of enzyme immunoassay (EIA) for IgE may yield variable results based on the patient's allergy status and serum IgE levels. Cross-reactivity with other Cucurbitaceae family members or non-specific binding in the sample may lead to increased false positives or variable result interpretation. The test results should be considered in conjunction with other clinical findings and patient history. Grossly lipemic samples are rejected as they can interfere with the assay, causing inaccurate results.
Methodology
Immunoassay (ELISA)
Biomarkers
LOINC Codes
- 7718-0
- 7718-0
- 102824-0
Result Turnaround Time
4-7 days
Related Documents
For more information, please review the documents below
Specimen
Serum
Volume
0.5 mL
Minimum Volume
Not provided
Container
Preferred: Red top; Acceptable: Serum gel. Submission: Plastic vial.
Collection Instructions
Centrifuge and aliquot 0.5 mL of serum into a plastic vial. Send refrigerate.
Storage Instructions
Refrigerated (preferred) or ambient for up to 28 days. Frozen samples can be stored for 365 days.
Causes for Rejection
Gross lipemia
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | 28 days |
| Refrigerated | 28 days |
| Frozen | 365 days |
