1p/19q Co-deletion (Oligodendroglioma), paraffin
Use
FISH testing on paraffin-embedded cells is used for the detection of deletions of chromosome arms 1p and 19q. These deletions are frequent genetic abnormalities in primary human gliomas and are especially common in oligodendrogliomas. This provides important genetic information that can influence diagnosis and treatment planning.
Special Instructions
Tissues should be fixed in 10% neutral buffered formalin (NBF). Tissues preserved in B+ fixative or decalcified are usually not suitable for FISH. The assay requires a minimum of three unstained slides (two for each probe) and one H&E stained slide. If unstained slides are sent, cut FFPE tissue sections at 3-5 μm thickness and mount them on positively charged slides.
Limitations
The analysis is not suitable for tissues preserved in B+ fixative or that have been decalcified. It requires proper fixation and sufficient tissue quantity to ensure reliable results. Extreme temperatures during shipment can affect the integrity of the specimen. Assay results may be impacted if minimum slide requirements are not met.
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
Result Turnaround Time
9 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
Slide
Storage Instructions
Store between 15°C to 25°C.
Causes for Rejection
Improper fixative; Tissue not fixed; Insufficient tissue; Specimen exposed to extreme temperatures
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Stable indefinitely |