ALK 2p23 Rearrangement, paraffin
Use
FISH testing on paraffin-embedded cells for detection of ALK (2p23) gene rearrangement is important for identifying patients with ALK-rearranged non-small cell lung cancer (NSCLC). Such patients may benefit from treatment with targeted tyrosine kinase inhibitors. ALK rearrangements are significant in the pathology of NSCLC and are often tested alongside ROS1 rearrangements to guide treatment planning.
Special Instructions
Specimens should be fixed in 10% neutral buffered formalin before submission. Tissues preserved in B+ fixative or decalcified are not suitable for FISH analysis. Assay requires a minimum of 3 unstained slides, with two required for each probe and one H&E stained slide. All slides need to be cut at 3-5 μm and mounted on positively charged slides.
Limitations
The test is limited by the fixation methods; tissues not fixed in 10% neutral buffered formalin or that are decalcified and preserved in B+ fixative cannot be tested. The test also requires sufficient tissue samples: a minimum of three unstained slides are necessary along with specific conditions being met for the FFPE tissues.
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
Result Turnaround Time
5 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
3 slides minimum
Container
Positively charged slides
Collection Instructions
Cut FFPE tissue sections at 3-5 μm thickness, mounted on positively charged slides.
Storage Instructions
Store specimens at 15°C to 25°C.
Causes for Rejection
Improper fixative; Tissue not fixed; Insufficient tissue; Specimen exposed to extreme temperature
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Stable indefinitely |