Alpha Fetoprotein, technical only
Use
AFP (alpha fetoprotein) is normally expressed during embryonic development and is found in the yolk sac and fetal liver. It can also be abnormally expressed in tumors such as hepatocellular carcinoma and germ cell tumors. This test uses immunohistochemistry to detect the presence of AFP in tissue samples.
Special Instructions
Technical component only; pathologist read not included. Testing is performed by an affiliate laboratory. For fresh tissues, use a 10% neutral buffered formalin fixative to tissue ratio of 20:1. For unstained slides, use positively charged glass slides to prevent background staining and tissue loss. Samples must be tested within 3 months if stored in ethanol.
Limitations
This test is technical only and does not include the pathologist's interpretation. Stability for fresh tissue is up to 72 hours. After fixation, specimens can be transferred to 70% ethanol for transportation, but this is not preferred for long-term storage. Samples stored in ethanol must be tested within 3 months.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
7 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin for 6-72 hours (48-72 hours preferred).
Storage Instructions
Store at 15°C to 25°C. For long-term storage, specimens may be stored at 2-8°C in 70% ethanol after fixation. Specimens must be tested within 3 months.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |