BCL6 3q27 Rearrangement, paraffin
Use
FISH testing on paraffin-embedded cells for detection of BCL6 (3q27) gene rearrangement. BCL6 rearrangements occur in diffuse large B-cell lymphoma, follicular center cell lymphoma, and marginal zone B-cell lymphoma. Often ordered as part of the FP BURKITT Panel test. These rearrangements are crucial for diagnosing and assessing the prognosis of these lymphoma types.
Special Instructions
Tissues should be fixed in 10% neutral buffered formalin (NBF). Tissues preserved in B+ fixative or decalcified are usually not suitable for FISH. Assay requires 3 unstained slides minimum (two for each probe) and one H&E stained slide. Ensure FFPE tissue sections are cut at 3-5 μm thickness and mounted on positively charged slides.
Limitations
Specimen stability is considered indefinite, but improper fixation, insufficient tissue, or exposure to extreme temperatures can lead to rejection. Tissues preserved in B+ fixative or decalcified may not be suitable for analysis. The analysis can be affected by tissue preservation methods, and extreme shipping conditions can compromise specimen integrity.
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
Result Turnaround Time
5 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
FFPE Tissue Block or Unstained FFPE tissue on 5 slides preferred (3 slides minimum) cut at 3-5 μM and mounted on positively charged slides.
Storage Instructions
Store at 15°C to 25°C.
Causes for Rejection
Improper fixative; Tissue not fixed; Insufficient tissue; Specimen exposed to extreme temperature
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Stable indefinitely |