BCR/ABL1 qRT PCR, major and minor
Use
Combined quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and reporting of the major and minor BCR::ABL fusion gene transcripts p210 (e13/e14a2) and p190 (e1a2) in patients with a Philadelphia (t(9;22)(q34;q11.2)) positive leukemia.
Special Instructions
Not provided.
Limitations
Specimen must be received by MPLN within 48 hours of collection. Specimen stability at 2-8°C; refrigerated shipping with ice pack is recommended. Specimens >48 hours old, clotted, stored or shipped at incorrect temperatures, or in incorrect anticoagulant, or of insufficient volume may be rejected; isolated RNA of suboptimal quality/quantity is also a criterion for rejection.
Methodology
PCR-based (qRT-PCR)
Biomarkers
BCR-ABL1 fusion
Fusion
Result Turnaround Time
7 days
Related Documents
For more information, please review the documents below
Specimen
Whole Blood
Volume
10 mL
Minimum Volume
5 mL
Container
EDTA preferred (NaHep accepted)
Storage Instructions
Specimen stability at 2-8°C
Causes for Rejection
Specimen >48 hours old; clotted; stored at incorrect temperature; incorrect anticoagulant; insufficient volume; RNA quality