BRAF V600E, technical only
Use
BRAF V600E is a protein marker targeting a mutation in the BRAF gene, substituting valine with glutamic acid at position 600, activating the MAPK signaling pathway, and driving tumorigenesis. It serves as a diagnostic and prognostic marker for cancers including melanoma, colorectal cancer, Hairy cell leukemia, and thyroid cancer. This test is technical only, with pathologist read not included.
Special Instructions
Not provided.
Limitations
The test does not include pathologist interpretation. Specimens must be fixed in 10% NBF or B plus; all other fixatives will be rejected. Turnaround times are subject to change based on biopharma protocol requirements and the final TAT is specified in the biopharma study contract.
Methodology
Immunoassay (IHC)
Biomarkers
BRAF V600E
Protein
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin for 6-72 hours (48-72 hours PREFERRED).
Storage Instructions
Store at ambient temperature, 15°C to 25°C.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |