Burkitt/'Double Hit' Large B-cell Lymphoma Panel, paraffin
Use
FISH Panel testing on paraffin-embedded cells for the most common abnormalities observed in Burkitt and high-grade B-cell lymphoma. The test identifies specific genomic rearrangements that can aid in the diagnosis and classification of these lymphomas.
Special Instructions
Tissues should be fixed in 10% neutral buffered formalin. Tissues preserved in B+ fixative or decalcified are usually not suitable for FISH. Assay requires a minimum of 5 unstained slides and one H&E stained slide.
Limitations
The test is specifically designed for detecting c-MYC, BCL6, IGH::MYC, and IGH::BCL2 rearrangements. Inadequate tissue fixation and improper tissue handling may affect results. Extreme temperatures during shipping can cause specimen rejection.
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
Result Turnaround Time
5 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
positively charged slides
Collection Instructions
Use FFPE tissue block or unstained FFPE tissue with 7 slides preferred (5 slides minimum) cut at 3-5 μM and mounted on positively charged slides.
Storage Instructions
15°C to 25°C
Causes for Rejection
Improper fixative; Tissue not fixed; Insufficient tissue; Specimen exposed to extreme temperature
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Stable indefinitely |