C MYC 8q24 Rearrangement, paraffin
Use
FISH testing on paraffin-embedded cells for detection of c-MYC (8q24) gene rearrangement. c-MYC is frequently rearranged in aggressive B-cell lymphomas, including Burkitt’s lymphoma. Often ordered as part of the FP BURKITT Panel test.
Special Instructions
Tissues should be fixed in 10% neutral buffered formalin (NBF). Tissues preserved in B+ fixative or decalcified are usually not suitable for FISH. Assay requires 3 unstained slides minimum (two for each probe) and one H&E stained slide. If unstained slides are sent, cut FFPE tissue sections at 3-5 μm thickness, mounted on positively charged slides.
Limitations
The test is limited by the fixation and embedding quality of the tissue sample. Specimens preserved in B+ fixative or decalcified are generally unsuitable for analysis. The assay requires a minimum of three unstained slides and may be impacted by insufficient tissue quantity or quality.
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
Result Turnaround Time
5 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
5 slides preferred (3 slides minimum)
Container
positively charged slides
Collection Instructions
FFPE Tissue Block or unstained FFPE tissue; cut sections at 3-5 μm thickness, mounted on positively charged slides.
Patient Preparation
Tissues should be fixed in 10% neutral buffered formalin.
Storage Instructions
Store at 15°C to 25°C
Causes for Rejection
Improper fixative; Tissue not fixed; Insufficient tissue; Specimen exposed to extreme temperature
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Stable indefinitely |