CD10, CALLA
Use
Cluster of differentiation 10 (CD10) is critical for use in the diagnosis and subclassification of leukemias (e.g., B lymphoblastic leukemia, mixed phenotype acute leukemia, etc.) and lymphomas (e.g., follicular lymphoma, Burkitt lymphoma, diffuse large B cell lymphoma, angioimmunoblastic T cell lymphoma).
Special Instructions
Fresh Tissues: Specimens may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. Samples must be tested 3 months after placing in ethanol. Unstained slides: Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.
Limitations
The test is subject to limitations based on the type of specimen and fixation method used. Only specimens fixed in 10% neutral buffered formalin (NBF) or B plus are accepted. Other fixatives will result in specimen rejection.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio; use 10% neutral buffered formalin for 6-72 hours (48-72 hours preferred).
Storage Instructions
Store at 15°C to 25°C. Ambient shipping conditions recommended with refrigerated (not frozen) gel pack to protect from extreme temperatures.
Causes for Rejection
Specimens fixed in non-approved fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |