CD5, technical only
Use
CD5 is a transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes, and some B lymphocytes (B1a). It can aid in diagnosing lymphomas and thymic carcinoma and in differentiating chronic lymphocytic leukemia (CLL) and mantle cell lymphoma (MCL) from other types of low-grade B-cell non-Hodgkin lymphomas. This is a technical component only, with no pathologist read included.
Special Instructions
Fresh tissues may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer-term storage if needed. Unstained slides require positively charged glass to prevent background staining and tissue loss. Slides should remain unbaked.
Limitations
The test is purely technical with no pathologist interpretation included. Specimens must be fixed in 10% NBF or B plus, as other fixatives will be rejected. Stained and unstained slides have indefinite stability, while fresh tissues are stable for up to 72 hours.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 ratio, 10% neutral buffered formalin for 6-72 hours (48-72 hours preferred).
Storage Instructions
Store at 15°C to 25°C.
Causes for Rejection
Specimens fixed in other than 10% NBF or B plus will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |