Cyclin D1/PRAD1
Use
Cyclin D1 is a cell cycle regulator that controls transition from G1 to S phase in normal tissues. A mutation in this protein leads to increased cell proliferation via disruption of downstream pathways, resulting in tumorigenesis. Detection by immunohistochemistry is valuable in the diagnosis and prognosis in a number of tumors including mantle cell lymphoma.
Special Instructions
Fresh tissues should be fixed at a 20:1 fixative to tissue ratio using 10% neutral buffered formalin for 6-72 hours. Unstained slides must use positively charged glass to prevent background staining and tissue loss, and should be left unbaked.
Limitations
Specimens fixed in any other fixative than 10% NBF or B plus will be rejected. The samples must be tested within 3 months after being placed in ethanol.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
FFPE Block
Storage Instructions
Store at 15°C to 25°C.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinite |