E-cadherin, technical only
Use
E-cadherin (ECAD) is a transmembrane protein expressed on almost all epithelial cells and involved in cellular adhesion and polarity maintenance. Loss of E-cadherin expression is associated with gain of tumor cell motility and invasiveness. This marker may be used to differentiate invasive and in situ / invasive lobular carcinoma (-) from in situ / invasive ductal carcinoma (+) of the breast. Technical component only. Pathologist read not included.
Special Instructions
Fresh Tissues: Specimens may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. Samples must be tested 3 months after placing in ethanol. Unstained slides: Positively charged glass slides required to prevent background staining and prevent tissue loss. Slides should be left unbaked.
Limitations
Stained slides and unstained slides have indefinite stability under recommended storage conditions. Specimens not fixed in 10% NBF or B plus will be rejected. Turnaround times are subject to change based on biopharma protocol requirements.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fresh)
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED).
Storage Instructions
15°C to 25°C. Use a refrigerated (NOT FROZEN) gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen.
Causes for Rejection
Specimens must be fixed in 10% NBF or B plus. All other fixatives will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |