E3 ubiquitin-protein ligase, technical only
Use
E3 ubiquitin-protein ligase mediates ubiquitination of p53, leading to its degradation by the proteasome. Amplified E3 ubiquitin-protein ligase is found in many tumors, leading to suppression of p53 in tumor types such as atypical lipomatous tumor, well-differentiated liposarcoma, and de-differentiated liposarcoma.
Special Instructions
Technical component only. Pathologist read not included. Fresh tissues may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed.
Limitations
Specimens must be tested within 3 months after placing in ethanol. Positively charged glass slides are required to prevent background staining and prevent tissue loss. Slides should be left unbaked. Specimens that are not fixed in 10% NBF or B plus will be rejected.
Methodology
Immunoassay (IHC)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
Not provided
Container
FFPE Block, Formalin Fixed
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours PREFERRED). Specimens may be transferred to 70% alcohol for transportation after fixation in 10% NBF or B plus.
Storage Instructions
Store at 15°C to 25°C. Use a refrigerated gel pack in the shipment to protect from extreme temperatures. Separate gel pack from specimen.
Causes for Rejection
Specimens not fixed in 10% NBF or B plus.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Indefinite |