EGFR 7p12 Amplification, paraffin
Use
FISH testing on paraffin-embedded cells for detection of EGFR (7p11.2) gene amplification. Amplification of EGFR is a recurrent abnormality in a variety of cancers, including non-small cell lung cancer, metastatic colorectal cancer, and glioblastoma. The detection of EGFR amplification can help in the diagnosis and potential therapeutic targeting of such cancers.
Special Instructions
Tissues should be fixed in 10% neutral buffered formalin (NBF). Tissues preserved in B+ fixative or decalcified are usually not suitable for FISH. Assay requires 3 unstained slides minimum (two for each probe) and one H&E stained slide.
Limitations
Assay accuracy may be compromised if tissues are not properly fixed in 10% neutral buffered formalin (NBF). Tissues preserved in B+ fixative or decalcified are usually unsuitable for FISH analysis. Improper fixation and extreme temperatures can lead to specimen rejection. The test is not validated for tissues fixed with alternative methods.
Methodology
Chromosomal / Cytogenetics (FISH)
Biomarkers
Result Turnaround Time
9 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (FFPE)
Volume
Not provided
Minimum Volume
3 slides
Container
positively charged slides
Collection Instructions
Cut FFPE tissue sections at 3-5 μm thickness, mounted on positively charged slides. Use at least 3 unstained slides; two for each probe and one H&E stained slide.
Storage Instructions
Store at 15°C to 25°C.
Causes for Rejection
Improper fixative; Tissue not fixed; Insufficient tissue; Specimen exposed to extreme temperature
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | Stable indefinitely |