Epstein Barr Virus Early RNA
Use
EBER ISH is an in situ hybridization assay designed to stain latent Epstein-Barr virus (EBV) in tissues. It provides valuable information for diagnosing EBV-related diseases by identifying viral RNA in tissue samples through specific staining techniques.
Special Instructions
Fresh Tissues: Specimens may be fixed in formalin for 24-72 hours by collection sites, then transferred to 70% ethanol and stored at 2-8°C for longer term storage if needed. Unstained slides should be left unbaked to prevent background staining and tissue loss.
Limitations
Specimens must be fixed in 10% NBF or B plus as all other fixatives will be rejected. The performance and accuracy of the assay may be affected by improper fixation, incorrect specimen handling, or environmental conditions not maintained as recommended.
Methodology
Chromosomal / Cytogenetics (ISH)
Biomarkers
Result Turnaround Time
2 days
Related Documents
For more information, please review the documents below
Specimen
Tissue (Fixed (Non-FFPE))
Volume
Not provided
Minimum Volume
Not provided
Collection Instructions
Fixative should be at a 20:1 fixative to tissue ratio, 10% neutral buffered formalin (NBF) for 6-72 hours (48-72 hours preferred). May transfer to 70% alcohol for transportation after fixation.
Storage Instructions
Store at 15°C to 25°C for fresh tissues; long-term storage in 70% ethanol at 2-8°C is an option post-fixation.
Causes for Rejection
All other fixatives apart from 10% NBF or B plus will be rejected.
Stability Requirements
| Temperature | Period |
|---|---|
| Room Temperature | ≤ 72 Hours |